Analysis of luteinizing hormone (LH): Validation of a commercial ELISA kit for LH analysis and quantification in doping control samples.
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Analysis of luteinizing hormone (LH): Validation of a commercial ELISA kit for LH analysis and quantification in doping control samples.
Luteinizing hormone (LH) is a glycoprotein dimer is produced and secreted by the pituitary gland, with a molecular weight of about 30 000 Da. The main clinical use of exogenous LH-administration normally associated with the treatment of infertility in both men and women. The desired effect of LH misuse in sport is due to increased testicular production of testosterone. The increase in LH levels may also indicate the use of gonadotropin-releasing factor or estrogen blocker.
Therefore, LH is listed by the World Anti-Doping Agency (WADA) as a banned substance in male athletes, and according to the technical documents WADA, the laboratory shall determine the total LH concentration in the urine. The TD list two different tests are suitable for measuring the amount of LH in the urine, Delfia and Siemens Immulite. fit-for-purpose test may be used, provided they are able to detect the amount of LH in the urine. In the case of the assay is not listed in the TD will be used, population-based reference values should be determined in the validation procedure.
In this study, a new immunoassay approved for measurement of LH in the urine. Assay (ELISA microwell AccuBind, luteinizing hormone, Monobind Inc.), originally intended for the serum, indicating adequate sensitivity and proven fit-for-purpose in a routine doping control. distribution of a population-based assay was in good agreement with the results of Delfia and Immulite test, which is a special method of cut-off-value of 40 IU / L (Delfia) and 60 IU / L (Siemens Immulite).
Analysis of luteinizing hormone (LH): Validation of a commercial ELISA kit for LH analysis and quantification in doping control samples.
Monitoring of trough levels of infliximab and anti-infliximab antibody for inflammatory bowel disease: A comparison of three commercially available ELISA kit.
There are many studies presenting biological data and some commercially available ELISA kit for monitoring the serum infliximab through rate (s-IFXt) and anti-drug antibodies (adab). We propose to compare the technical characteristics and the results of three different tests in a cohort of 35 patients under infliximab (IFX) and suffering from inflammatory bowel disease (IBD) .S-IFXt and manners are systematically measured by the three ELISA kits: Lisa-Tracker Duo infliximab (Theradiag®), Ridascreen® IFX Monitoring (R-Biopharm AG®) and Promonitor® IFX (Progenika Biopharma SA®) .the main technical features that differ between the kit for measuring s-IFXt are: (i) TNF coating, (ii ) revelation complex immune strategy and / or (iii) interference with anti-TNF agents other.
For measuring kit courtesy, they step revelation and unit results. There is a very good mathematical correlation of s-IFXt between tests But Bland-Altman analysis denoted by (i) s-IFXt were on average 48-69% higher in Ridascreen® than two other tests, and (ii) an increase in s- IFXt higher with Promonitor® compared to Lisa-Tracker. As a result, there are some major differences between test for classification of s-IFXt into concentration range. Although the unit unstandardized, qualitatively Pairwise Comparisons show a perfect agreement between the three pairs of data courtesy assays.
Description: A competitive ELISA for quantitative measurement of Canine major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativecompetitive ELISA kit for measuring Pig major acute phase protein (MAP) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativecompetitive ELISA kit for measuring Pig major acute phase protein (MAP) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive ELISA for quantitative measurement of Porcine major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig major acute phase protein (MAP) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea Pig major acute phase protein (MAP) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea Pig major acute phase protein (MAP) ELISA kit
Our show that the test is evaluated quantitatively not interchangeable due to substantial variation in some of the results may lead, for some patients, for divergent therapeutic decisions. We are reminded to be careful when comparing results of the study were excluded from different kits and suggested using the same assay for longitudinal follow-up of patients with IBD.
