HEV seroprevalence in blood donors in Turkey by two commercial total anti-HEV Ab ELISA kits.
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HEV seroprevalence in blood donors in Turkey by two commercial total anti-HEV Ab ELISA kits.
Previous studies the prevalence of hepatitis E virus (HEV) in Turkey have shown high variability, leading to conflicting results. We aim to re-evaluate the HEV prevalence among blood donors in Turkey using Wantai (Beijing, China) and Dia.Pro (Milan, Italy) Total anti-HEV antibody (Ab) enzyme-linked immunosorbent assay (ELISA) kit and compare their performance and to investigate the presence of HEV RNA in blood donors. Total serum anti-HEV antibody is determined in a total of 2011 samples of voluntary blood donations collected from various regions of Turkey (807 from Ankara, 243 of Kayseri, 284 of İzmir, 200 of Malatya, Kahramanmaras 200, and 277 of Van). HEV RNA was evaluated by real-time polymerase chain reaction in a total of 272 anti-HEV seropositive samples.
A country-wide HEV seroprevalence was calculated as 11.5% (Dia.Pro) and 12.2% (Wantai) with seropositivity rate of 12.0% -12.5% in Ankara, 7.4% -8.2% in Kayseri, 14.5% -15.5% in Malatya, 8.1% -8.8% in Izmir, 15.0% -16.0% in Kahramanmaras, and 12.6% -13.4% in Van by Dia.Pro and Wantai kit, respectively. Ab lowest concentration detected was 0.16 and 0.14 units / mL WHO, for Dia.
Pro and test Wantai, respectively, showed no significant differences between the tests. HEV RNA was not detected in samples of anti-HEV seropositivity. Compared with previous studies, HEV shown to have a higher overall seroprevalence in Turkey. Despite its limitations, this study is the most comprehensive study of HEV seroprevalence in Turkey conducted by two different commercial ELISA test with high sensitivity so far. Further investigation is needed to determine the genotypes of HEV in Turkey.
HEV seroprevalence in blood donors in Turkey by two commercial total anti-HEV Ab ELISA kits.
Comparison of Two Commercial ELISA Kit for Detection of Anti-Dengue Dengue IgM for routine diagnosis in Laos.
The endemicity of dengue virus (DENV) infection remains a major public health problem in Lao PDR. In this study, we compared two commercial anti-dengue IgM ELISA kit, Panbio® Dengue IgM Capture ELISA (Panbio Kit, Alere, Waltham, MA, USA) and DEN DetectTM MAC-ELISA (InBios kit, InBios International, Inc., Seattle , WA, USA), in the context of the diagnosis of patients hospitalized with dengue clinical presentation. Two panels of paired blood samples tested.
A panel composed of 54 dengue patients confirmed (by DENV real-time RT-PCR) and 11 patients with non-dengue hemorrhagic fever (another infection confirmed by appropriate PCR). Panel B included 74 patients who were randomly selected from consecutive patients hospitalized Mahosot in 2008 with suspected dengue fever according to WHO criteria.
Results of panel A shows a significantly better sensitivity for Panbio kit (64.8%; 95% CI: 50.6 to 77.3%) compared InBios kit (18.5%; 95% CI: 9.3 to 31 , 4%) when testing sera entry. Increased sensitivity to both the kit when combining the results of the entrance and the sera recovered. The results obtained from the panel B in accordance with a fair agreement (κ = 0.29) between the two kits when testing a single sample entry, and deal moderate (κ = 0.5) when combining the results of the entrance and the sera recovered.
Prolactin has been reported to be an index of the tremendous response to stress, both acute and chronic, in some species. Use of the biological matrix in addition to the blood receiving increasing interest in the study of hormones, because invasive lower in the collection.
Description: Description of target: As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.;Species reactivity: Cat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 4.261 ng/ml
Description: A sandwich ELISA for quantitative measurement of Rat Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Guinea pig Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Guinea pig Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Guinea pig Haptoglobin Related Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey HPR (Haptoglobin Related Protein) ELISA Kit
This study aims to investigate the possibility of using ELISA (enzyme-linked immunosorbent assay) a commercial kit to measure canine prolactin in the blood for the quantification of prolactin in the saliva of the dog. Study 1 consisted of a validation protocol, using saliva samples collected from lactating and non-lactating dogs.
