Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle.
»
Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle.
Fasciola hepatica is the mammalian liver parasite and it produces the result of poor welfare and economic losses in ruminants. While the number of eggs stool is the most common test used for diagnosis, it does not indicate a migration of the adult stage. serological techniques improve the sensitivity at all stages of liver fluke infection. The purpose of this study was to compare four commercially available ELISA test for the diagnosis of F. hepatica.
To this end, we tested three types of samples; (I) is known F. hepatica Status sera from experimental infection for the comparison of sensitivity and specificity, (ii) sera from pre- and post-flukicide-treated (albendazole, closantel, nitroxynil and triclabendazole) beef cattle seropositive for contrast difference before and after treatment, and (iii) samples of bulk tank milk samples from dairy cattle during periods of exposure to high and low F. hepatica to assess seasonal variation with four tests available.
Samples were assayed using ELISA kit provided by four manufacturers (Ildana Biotech, IDEXX, Svanova, and Bio-X). Samples were analyzed simultaneously and in duplicate.In Ildana population control, IDEXX and Bio-X presented 100% sensitivity (Se) and specificity (Sp), Svanovir served Se and Sp of 59% from 96%. In flukicide-treated beef, kit highlighted a decrease in antibody levels 90 days post-treatment in variable degrees.
Finally, bulk milk showed a significant decrease in the value of ELISA between periods of high and low exposure to coincidence with all tests and Sp studied.Se found in this study, confirming that Ildana, IDEXX and Bio-X accurate for detecting exposure to F. hepatica in cattle Ireland. Svanovir Se and Sp in this population, suggesting that larger studies are needed to confirm the characteristics of these tests in the herd of Ireland.
In the use of post-treatment, Bio-X showed a consistent and significant reduction of the value of the ELISA in all treatment groups, which were shown to be a reliable tool to assess the effects of treatment on day 90 post-treatment. Finally, all tests showed to be a reliable tool for monitoring F. hepatica high season and low exposure, using bulk tank milk samples.
Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle.
Improved diagnosis of diabetes type Japanese-onset autoimmune first time using a new ELISA kit GADAb, compared to the previously used GADAb RIA kit.
We compared the results of glutamic acid decarboxylase antibody test (GADAb) using radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) in subjects with age-onset diabetes mellitus type 1 specimen (T1D) .Serum collected from 1024 cases of Japan (426 laki- male and 598 female) in 2013. the average age of diagnosis was 7 years (0-18 years).
Blood specimen obtained at a mean age of 13 years (2-22 years) .Among 628 subjects whose serum specimens collected within 5 years after diagnosis, positive GADAb level is 47.9% and 69.4% using a RIA using ELISA. The subjects were divided into four groups according to their RIA and ELISA results for GADAb as follows: Gr I (RIA + / ELISA +), Gr II (RIA + / ELISA-), Gr III (RIA- / ELISA +) and Gr IV (RIA – / ELISA-).
Clinical characteristics and genetic Gr Gr I and III were very similar in terms of age at diagnosis, male / female ratio, a relatively high positive rate for both IA-2AB and ZnT8Ab, and HLA genotype. Gr II only contains 5 patients and is characterized by a younger age at diagnosis, a low positive rate for both IA-2AB and ZnT8Ab and unique HLA genotype.
BARX1 (Prognostic Biomarker in Hepatocellular Carcinoma)
Description: Her-2 IHC Bio-marker control TMA, with breast invasive ductal carcinoma, including TNM, clinical stage and pathology grade, 10 cases/ 10 cores, replaced by Her2b
Her-2 IHC Bio-marker control TMA, with breast invasive carcinoma of no special type, including pathology grade, TNM, clinical stage and IHC markers (ER, PR, Her-2 and Ki-67) results, 10 cases/10 cores, replacing Her2b
Description: Her-2 IHC Biomarker control tissue microarray of breast invasive carcinoma of no special type, containing 10 cases of invasive invasive carcinoma of no special type, single core per case
Rat Myosin-binding protein C, cardiac-type,Cardiac MyBP-C ELISA Kit
Description: A competitive ELISA for quantitative measurement of Rat Cardiac Troponin I in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cardiac Troponin I in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cardiac Troponin I in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cardiac Troponin T in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cardiac Troponin T in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cardiac Troponin T in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat cardiac Troponin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat cardiac Troponin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat cardiac Troponin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine cardiac Troponin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine cardiac Troponin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine cardiac Troponin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cardiac Troponin I in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cardiac Troponin I in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cardiac Troponin I in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cardiac Troponin T in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cardiac Troponin T in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cardiac Troponin T in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
×
If the positive rate of both IA-2AB or ZnT8Ab or both are added to the results GADAb using RIA, autoimmune T1D percentage increased from 47.9% to 78.5% .the diagnosis of autoimmune time-onset T1D Japan GADAb increased when the results obtained using the new ELISA method , compared with RIA method previously used.
