Multicenter Evaluation of the C6 Lyme ELISA Kit for the Diagnosis of Lyme Disease.
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Multicenter Evaluation of the C6 Lyme ELISA Kit for the Diagnosis of Lyme Disease.
Lyme disease (LD), caused by infection with Borrelia burgdorferi, is a tick-borne infection that is most common in many regions of Eurasia. detection of antibodies are laboratory tests are most often used, supports a two-step algorithm serodiagnostic; immunoenzymatic detection of antibodies to C6 has proven to perform similarly to the two-step standard workflow.
The purpose of this study is the evaluation of the performance of the C6 Lyme ELISA kit in comparison with a standard two-step algorithm in three laboratories located in the northeast region of Italy that is destined for areas with different epidemiological LD. A total of 804 samples tested, the 695 provides results in line between C6 and workflow testing routine (564 negative, positive 131).
Wherever available, the clinical presentation and additional laboratory tests are analyzed to resolve the differences. Methods based on C6 demonstrated good concordance with standard two-step algorithm (Cohen κ = 0.619), however, the distribution of difference seems to point towards a slightly lower specificity of the test C6, which is supported by the literature and could have an impact on patient management. C6 ELISA, therefore, not a stand-alone test that ideal; However, if it is integrated into a two-step algorithm, may be instrumental in achieving a sensitive, specific laboratory diagnosis of LD.
Multicenter Evaluation of the C6 Lyme ELISA Kit for the Diagnosis of Lyme Disease.
Development of Direct Competitive ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat.
This research was conducted to develop a competitive enzyme-linked immunosorbent assay (dcELISA) direct assembly kit for detecting deoxynivalenol (DON) in food and feed grains. Based on the preparation of monoclonal antibody anti-DON, we established a standard curve with dcELISA and optimized detection conditions.
Performance kit was evaluated in comparison to high-performance liquid chromatography (HPLC). The minimum detection limit of the kit DON with 0.62 ng / mL, the linear range is 1.0 to 113.24 ng / mL and a half maximal inhibitory concentration (IC50) was 6.61 ng / mL in buffer work; there is a limit of detection (LOD) of 62 ng / g, and the detection range is 100-11324 ng / g in the sample authentic farm. We checked out four samples of wheat bran, wheat flour, corn flour and corn for the recovery of DON. Average recovery was in the range of 77.1% to 107.0%, and relative standard deviation (RSD) ranged from 4.2% to 11.9%.
In addition, the kit has the advantages of high specificity, good stability, long life effective and overlooked sample matrix interference. Finally, a sample of grain from farms in the six provinces of Henan, Anhui, Hebei, Shandong, Jiangsu and Gansu in China are analyzed with the kit. A total of 30 samples were randomly checked (five samples in each province), and the results are in good agreement with standard HPLC method. These tests showed that the kit dcELISA has good performance and meet the relevant technical requirements, and has the characteristics of accuracy, reliability, convenience and high-throughput screening for the detection of DON. Therefore, advanced kit is suitable for rapid screening of DON in the product being marketed.
β-Conglycinin is the major storage proteins in soybeans. pre-clinical animal models and human clinical studies have shown triglyceride-lowering effect of these proteins, suggesting that it could be put into practical use as a functional food ingredient. To date, however, there is no accurate and simple test for the quantification of β-conglycinin. In this study, the sample pre-treatment by mixing them with flour before extracting protein rice flour.
Description: A competitive ELISA for quantitative measurement of Rabbit major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig major acute phase protein (MAP) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig major acute phase protein (MAP) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig major acute phase protein (MAP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Chicken C Reactive Protein (CRP) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Chicken C Reactive Protein (CRP) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit C Reactive Protein (CRP) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit C Reactive Protein (CRP) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Guinea pig C Reactive Protein (CRP) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Guinea pig C Reactive Protein (CRP) in samples from serum, plasma or other biological fluids.
Description: C-reactive protein (CRP) belongs to the pentaxin family. CRP is a secreted protein found in plasma. It can binds two calcium ions per subunit.CRP can promotes phagocytosis, bacterial capsular swelling, complement fixation and agglutination through its calcium-dependent binding to phosphorylcholine which expressed on the surface of dead or dying cells. It can activate the complement system via the C1Q complex. CRP can interact with DNA and histones. In addition, CRP may scavenge nuclear material released from damaged circulating cells.
Description: C-reactive protein (CRP) is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are noncovalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat respectively. CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL6, IL1 and glucocorticoids are the major inducer of the CRP gene. The physiological role of CRP is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system. CRP binds to phosphocholine on microbes and damaged cells and enhances phagocytosis by macrophages. Thus, CRP participates in the clearance of necrotic and apoptotic cells. CRP rises up to 50,000-fold in acute inflammation, such as infection. It rises above normal limits within 6 hours, and peaks at 48 hours. Its half-life is constant, and therefore its level is mainly determined by the rate of production. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.
Goat Steroidogenic acute regulatory protein ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Steroidogenic acute regulatory protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Steroidogenic acute regulatory protein ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Steroidogenic acute regulatory protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Steroidogenic acute regulatory protein ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Steroidogenic acute regulatory protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Steroidogenic acute regulatory protein ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Steroidogenic acute regulatory protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Steroidogenic acute regulatory protein ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Steroidogenic acute regulatory protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Steroidogenic acute regulatory protein ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Steroidogenic acute regulatory protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Steroidogenic acute regulatory protein ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Steroidogenic acute regulatory protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Steroidogenic acute regulatory protein ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Steroidogenic acute regulatory protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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Then, we used a commercially available ELISA kit for the detection of allergens that may be present in the residue contaminated soybeans. This enables highly accurate and reproducible quantitation of β-conglycinin content in some processed foods soybeans.
